Ancient T-M184 Samples

[aaronbee2010]

Hello all,

A while back, I offered to analyse ancient samples under T-M184 on behalf of the T-M184 Facebook group. The current samples I've analysed are I0797, DA125, I1781, I2512, I2514, I1707, I0795, KARS537 and ANI152. Are there any other samples I've missed here? Any input here is appreciated.

Kind Regards,

- Aaron

EDIT: I've just found this (https://anthrogenica.com/showthread.php?16648-Ancient-T-M184-samples-map) thread by vettor, so I'll start analysing these samples now. Have there been any new discoveries not mentioned on this thread that I could look at? Thanks again.

[vettor]

here are some

Collegno ( 1350 yBP - Early Medieval ) Longobard Period


CL23 ( 1310 -1380 yBP )
Phase: I
Y-DNA: T1a2b1a1-CTS6071 (x Y21207, CTS7303)
mtDNA: H
Strontium isotope: Non-local
Diet (d13C%0 / d15N%0): -19.6 / 8.0 (Low Animal Protein)
Age at Death: Adult
Coverage: 2,952
Other IDs: COL001
Files: FASTQ / FASTQ&BAM (galaxy) / BAM (FASTQ=>mapped-BAM)


......................................

Szólád ( Early Medieval ) Lombard Period


SZ36
Y-DNA: T1a1a1a1a2b2-PF7275
mtDNA: U4c2a
Strontium Isotope: Non-local
Diet (d13C%0 / d15N%0): -18.4 / 11.6 (Highest Animal Protein)
Age at Death: Child
Coverage: 6,81
Other IDs: SZO031
Files: FASTQ / FASTQ (galaxy) / BAM (FASTQ=>mapped-BAM)


........................................


Varna ( 6500 yBP - Chalcolithic ) Varna Culture

ANI152/Varna43 ( 6495 yBP )


Y-DNA: T-Y3804 (x T1-Y3813)
mtDNA: U2
Age at Death: 50-65

......................................


I1108 ( 7550 ± 200 yBP )

House: 4
Y-DNA: T1a1a2-Y63197
mtDNA: T2e
Age at Death: Juvenile


...................................

Ipatovo 3 ( 5400 yBP - Early Bronze Age ) Early Steppe Maykop Culture

IV3002 ( 5383 ± 64 yBP )
Kurgan: 2
Phase: 1st
Grave: 187 ( Founding grave of the entire mound )
Other dates: 5058 ± 223 yBP / 5328 ± 251 yBP / 4630 ± 50 yBP (radiocarbon)
Y-DNA: T1-CTS6004 (xT1a1a-CTS484, T1a1b-Y6031, T1a2b-FGC37316) Probably: T1a2a-Y8614
mtDNA: X1'2'3
Age at Death: 35-45


......................................

Varnhem ( 950 yBP - Viking Age )


VK398 ( 950 ± 100 yBP )
House/Phase:
Y-DNA: T1a1b2a
mtDNA: H1b1+16362
Sr isotope:
Diet (d13C%0 / d15N%0):
Age at Death:
Death Position:
Other ID: 398, Sweden_Skara 231
Sample: Petrous




I will send you more later if you like

[aaronbee2010]

Thank you very much! I've also just seen your thread on ancient T-M184 samples (after starting this thread unfortunately!).

If you can send more later that would be great. The admin of PhyloGeographer has given me credential for upload these ancient SNP calls to his website to add to the Mygrations subclade path calculator, so the more samples the better :)

I'll get a start on these samples (as well as the ones in the map on your thread) in the meantime. Thanks again!

[vettor]

I ran in gedmatch a sample of neolithic (Germany ) Karsdorf T1a sample with neolithic Bulgaria T1a sample and they are nearly Identical


Comparing Kit Z017041 (I0700 Malak Preslavets) [Migration - F2 - Z] and Kit M671535 (I0795_Karsdorf) [Migration - V3 - M]



Largest segment = 38.4 cM

Total Half-Match segments (HIR) 914.7cM (25.716 Pct)

103 shared segments found for this comparison.

13541 SNPs used for this comparison.

89.609 Pct SNPs are full identical

[vettor]

Ran myself with other T1a neolithic Bulgarian sample and have all 22 chr have matching segments

me and Kit Z113504 (I1108 Malak Preslavets) [Migration - F2 - Z]


Largest segment = 19.5 cM

Total Half-Match segments (HIR) 1543.6cM (43.142 Pct)

320 shared segments found for this comparison.

73036 SNPs used for this comparison.

57.922 Pct SNPs are full identical

[vettor]

Karsdorf ( 7100yBP - Early Neolithic ) LBK Culture

I0795 ( 7076 ± 90 yBP )
House/Phase: S / 2
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: H1 or H1au1b*
Sr isotope: Native to Unstruttal
Diet (d13C%0 / d15N%0): -20.0 / 9.0 (highest Animal Protein)
Age at Death: 45-60
Death Position: Flexed Left northeast
Other ID: KAR6/Feature 170/Musm.no. 2006:14423a
Sample: Tooth / Rib
Read Pairs:
Mean Coverage:
Files: FASTQ / FASTQ&BAM (galaxy) / BAM




I0797 ( 7087 ± 725 yBP )
House/Phase: H / 2
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: H46b
Sr isotope: Native to Unstruttal
Diet (d13C%0 / d15N%0): -20.2 / 9.1 (highest Animal Protein)
Age at Death: 24-26
Death Position: Stretched Dorsal northwest
Other ID: KAR16a/Feature 611/Musm.no. 2004:26374a
Sample: Tooth / Rib
Read Pairs:
Mean Coverage:
Files: FASTQ / FASTQ&BAM (galaxy) / BAM



Kars537 ( 6958 ± 49 yBP )


House/Phase: O / 2
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: J1c6a
Sr isotope: Native to Unstruttal ( Rang Group limit )
Diet (d13C%0 / d15N%0): -19.7 / 8.9 (highest Animal Protein)
Age at Death: 25–30
Death Position: Stretched Dorsal southeast
Other ID: Feature 537 / Museum no 2004:26340a / 14C id KIA-40357
Sample: Skull
Read Pairs: 122,568,310
Mean Coverage: 2.96X
Virus: Hepatitis B ( HBV )
Autosomal notes: High CHG
Files: FASTQ / FASTQ (galaxy) / BAM (FASTQ=>mapped-SAM=>sorted BAM)

Karsdorf settlement


https://www.nature.com/articles/nature14317
doi: https://doi.org/10.1038/nature14317

https://elifesciences.org/articles/36666
doi https://doi.org/10.7554/eLife.36666.001

More research:
https://www.researchgate.net/publica...ntial_mobility








Malak Preslavets ( 7600yBP - Mesolithic/Early Neolithic ) Criș Culture

I0700 ( 7550 ± 200 yBP )

House: 13
Y-DNA: T1a1a2-Y63197
mtDNA: T2e
Age at Death: Adult
Death position: Disturbed grave. Perhaps was a flexed burial on the right side
Sample: Left Femur
Burial: Cattle bone was discovered next to the feet
Autosomal notes: The highest EHG+WHG+CHG in the settlement
Files: FASTQ / FASTQ&BAM (galaxy) / BAM




I1108 ( 7550 ± 200 yBP )

House: 4
Y-DNA: T1a1a2-Y63197
mtDNA: T2e
Age at Death: Juvenile
Death position: Crouched on the right side, with head to west
Sample: Left Tibia
Burial: Freshwater mussel shells
Autosomal notes: The highest EHG+WHG+CHG in the settlement
Files: FASTQ / FASTQ&BAM (galaxy) / BAM




Malak Preslavets settlement


https://www.nature.com/articles/nature25778

doi: https://doi.org/10.1101/135616



both these neolitic sites of germany and bulgaria with ancient T1a samples also have ancient samples of g2a-u8 found as well ........................with the new findings of 3 x t1a and the 3 x g2a-u8 found in North-east italy recently , then the conclusion is they all moved together in Europe

[vettor]

part of post #6

Kars537 ( 6958 ± 49 yBP )


House/Phase: O / 2
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: J1c6a
Sr isotope: Native to Unstruttal ( Rang Group limit )
Diet (d13C%0 / d15N%0): -19.7 / 8.9 (highest Animal Protein)
Age at Death: 25–30
Death Position: Stretched Dorsal southeast
Other ID: Feature 537 / Museum no 2004:26340a / 14C id KIA-40357
Sample: Skull
Read Pairs: 122,568,310
Mean Coverage: 2.96X
Virus: Hepatitis B ( HBV )
Autosomal notes: High CHG
Files: FASTQ / FASTQ (galaxy) / BAM (FASTQ=>mapped-SAM=>sorted BAM)


the first one that has been added by YFull

https://i.postimg.cc/XqzkQSWQ/537.png (https://postimages.org/)

[Jack Johnson]

Wow, so like H2, J2a, and E1b1b, T1a also accompanied G2a farmers into Europe. Kars537 also has CHG/Iran_N related admixture, which was also found in Otzi and in the AAFs (Anatolian Aceramic Farmers). It appears the E1b1b EEFs carried some Levant_N admixture; did some of the T1a ANFs/EEFs carry it as well? I wonder how many European T clades can be linked to Neolithic—Bronze Age movements. Since both H and T are present in Neolithic—Chalcolithic Europe, I’m curious to see if we’ll ever get E-M123, L, J2b, or J1 clades. Considering J2b was found in Nuragic samples from Sardinia, and those samples had practically no CHG, it is likely we'll get a few EEFs with J2b. L too, considering the fact that there are some pretty old clades with ancient TMRCA dates, dispersed throughout Europe. L and T are very close and share a parent clade, like R1a and R1b, J2a and J2b, and R and Q, so it would not be too surprising to see L tagging along with H2, G2a, and T1a.

[vettor]

Wow, so like H2, J2a, and E1b1b, T1a also accompanied G2a farmers into Europe. Kars537 also has CHG/Iran_N related admixture, which was also found in Otzi and in the AAFs (Anatolian Aceramic Farmers). It appears the E1b1b EEFs carried some Levant_N admixture; did some of the T1a ANFs/EEFs carry it as well? I wonder how many European T clades can be linked to Neolithic—Bronze Age movements. Since both H and T are present in Neolithic—Chalcolithic Europe, I’m curious to see if we’ll ever get E-M123, L, J2b, or J1 clades. Considering J2b was found in Nuragic samples from Sardinia, and those samples had practically no CHG, it is likely we'll get a few EEFs with J2b. L too, considering the fact that there are some pretty old clades with ancient TMRCA dates, dispersed throughout Europe. L and T are very close and share a parent clade, like R1a and R1b, J2a and J2b, and R and Q, so it would not be too surprising to see L tagging along with H2, G2a, and T1a.

Yes T ydna and L ydna came out of LT ydna ................these came out of K-M9 which is also father of R1a, R1b ydna group

one of the T Kars537 is also part of this investigation

https://elifesciences.org/articles/36666

[vettor]

Another T1a1 ydna sample from around the Harz Mountains Germany ..........neolithic time ................seems like the same linage as the 3 x Karsdorf T1a1 ydna neolithic samples

https://academic.oup.com/mbe/article/39/6/msac108/6586604?login=false

Abstract
Human expansion in the course of the Neolithic transition in western Eurasia has been one of the major topics in ancient DNA research in the last 10 years. Multiple studies have shown that the spread of agriculture and animal husbandry from the Near East across Europe was accompanied by large-scale human expansions. Moreover, changes in subsistence and migration associated with the Neolithic transition have been hypothesized to involve genetic adaptation. Here, we present high quality genome-wide data from the Linear Pottery Culture site Derenburg-Meerenstieg II (DER) (N = 32 individuals) in Central Germany. Population genetic analyses show that the DER individuals carried predominantly Anatolian Neolithic-like ancestry and a very limited degree of local hunter-gatherer admixture, similar to other early European farmers. Increasing the Linear Pottery culture cohort size to ∼100 individuals allowed us to perform various frequency- and haplotype-based analyses to investigate signatures of selection associated with changes following the adoption of the Neolithic lifestyle. In addition, we developed a new method called Admixture-informed Maximum-likelihood Estimation for Selection Scans that allowed us test for selection signatures in an admixture-aware fashion. Focusing on the intersection of results from these selection scans, we identified various loci associated with immune function (JAK1, HLA-DQB1) and metabolism (LMF1, LEPR, SORBS1), as well as skin color (SLC24A5, CD82) and folate synthesis (MTHFR, NBPF3). Our findings shed light on the evolutionary pressures, such as infectious disease and changing diet, that were faced by the early farmers of Western Eurasia


Individual DER031 belong to y haplogroup
T1a1
mtdna Hv+16311


Karsdorf samples are

Karsdorf ( 7100yBP - Early Neolithic ) LBK Culture

I0795 ( 7076 ± 90 yBP )
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: H1 or H1au1b*


I0797 ( 7087 ± 725 yBP )
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: H46b


Kars537 ( 6958 ± 49 yBP )
Y-DNA: T1a1a2-Y63197 (xBY154289)
mtDNA: J1c6a



The majority of other Ydna samples from the paper are G2a2 and H2