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kafky
04-30-2015, 08:18 AM
Hi to all!

This is my first post on this forum. I have a 23andme test that showed a H haplo, however doing James Lick test I found that my mtdna haplo is H100. I have contacted James Lick and he nicely confirmed the H100. I can not find much info about it since is a new one. However, I'd love to know others to help establishing a line for it.

My maternal line is Portuguese tracked from 1700 to today. Still no genealogical info before that. Any of you have more info on H100?

Thanks!

Kafky

GailT
04-30-2015, 02:50 PM
In the current Phylotree H100 is defined by A12358G which is a somewhat common mutation that has occurred independently at least twice in haplogroup H, so it is not reliable for defining a subclade of H, and the Phylotree needs to be revised for H100. If you have other extra mutations in mthap, they might help identify a new subclade of H, although you might need to test the full sequence to get a more definitive result.

Among the GenBank samples that currently define H100 there are 2 unrelated groups:

Samples from Scotland and Ireland have extra mutations at 93, 15110, 200, 1391 and 12358. I would change H100 to be defined by 93 and 15110 because there are other people who share these two mutations but who lack the mutation at 12358.

A GenBank sample from Austria has extra mutations at 1041, 9410, 12358, 13656, 16189 and 16192.

The full sequence test would determine if you are in one of these two groups or perhaps a new subclade of H.

kafky
04-30-2015, 04:32 PM
Thanks GailT! I am posting some results, it may clarify which group I am from. Here is the first match from mthap:


Best mtDNA Haplogroup Matches:

1) H100

Defining Markers for haplogroup H100:
HVR2: 263G
CR: 750G 1438G 4769G 8860G 12358G 15326G
HVR1:

Marker path from rCRS to haplogroup H100 (plus extra markers):
H2a2a1(rCRS) ⇨ 263G ⇨ H2a2a ⇨ 8860G 15326G ⇨ H2a2 ⇨ 750G ⇨ H2a ⇨ 4769G ⇨ H2 ⇨ 1438G ⇨ H ⇨ 12358G ⇨ H100 ⇨ 5048C 16242T (16519C)

Imperfect Match. Your results contained differences with this haplogroup:
Matches(6): 263G 750G 1438G 4769G 8860G 12358G
Extras(2): 5048C 16242T (16519C)
Untested(1): 15326

kafky
05-19-2015, 09:01 PM
In the current Phylotree H100 is defined by A12358G which is a somewhat common mutation that has occurred independently at least twice in haplogroup H, so it is not reliable for defining a subclade of H, and the Phylotree needs to be revised for H100. If you have other extra mutations in mthap, they might help identify a new subclade of H, although you might need to test the full sequence to get a more definitive result.

Among the GenBank samples that currently define H100 there are 2 unrelated groups:

Samples from Scotland and Ireland have extra mutations at 93, 15110, 200, 1391 and 12358. I would change H100 to be defined by 93 and 15110 because there are other people who share these two mutations but who lack the mutation at 12358.

A GenBank sample from Austria has extra mutations at 1041, 9410, 12358, 13656, 16189 and 16192.

The full sequence test would determine if you are in one of these two groups or perhaps a new subclade of H.



Hi again! Reviewing my raw mt and the two mutations 93 and 15110 I can see I have A for the first and G for the second. I do not know which results I should expect.
After contacting Mr James Lick he considers strongly a H100 result. However, things are always changing and new possibilities can be considered. Besides that, how can I get more info on this haplogroup? Seams so rare. Is it a new mutation?

Thanks for your detailed comment!

José

RCO
05-20-2015, 11:50 AM
In the case of mtDNA the best solution would be a FMS at FTDNA. You also would get your matches in the largest mtDNA available database.

kafky
05-20-2015, 03:59 PM
In the case of mtDNA the best solution would be a FMS at FTDNA. You also would get your matches in the largest mtDNA available database.

Caro amigo RCO

Thanks for your reply! However, the extra mutations defined by GailY are not "no calls" in the infamous 23andme test, so I have the results that I probably need, in this case. The question to GailT is what base should be presented on those snp's, A, C, G or T? Otherwise, H100 can be my mtdna haplo. Nevertheless, there is scarce information on H100.

Cumprimentos, do país irmão Portugal.

José

GailT
05-21-2015, 01:40 AM
Hi again! Reviewing my raw mt and the two mutations 93 and 15110 I can see I have A for the first and G for the second. I do not know which results I should expect.
After contacting Mr James Lick he considers strongly a H100 result. However, things are always changing and new possibilities can be considered. Besides that, how can I get more info on this haplogroup? Seams so rare. Is it a new mutation?

Thanks for your detailed comment!

José

The samples I listed above are 93G and 15110A so you don't match this group. 5048C is unique in H*, there is 1 sample with 16242T but it might be H3. So you would need the FMS to get a definitive result. It is also possible that you will define a new subclade in H.

kafky
05-21-2015, 03:46 PM
Thanks again GailT for your almost infinite patience! Then I should exclude the Scotland and Ireland branch. Yes I have 5048C and 16242T. Considering the possibility of the Austrian branch, the other snps results are: 1041A; 12358G; 13656T; 16189T; 16192C. Can you confirm or exclude a match with that branch?

Fortunately I have a very low level of "no calls" on mtDNA...

Warmest regards, José

kafky
05-25-2015, 12:02 PM
Citing: http://www.ianlogan.co.uk/sequences_by_group/h100_genbank_sequences.htm

CRS A263G A750G A1438G A4769G A8860G A15326G

H100 A12358G

1. HM625681 Kloss H100 07-DEC-2010 A263G 315.1C A750G A1041G A1438G A4769G A8860G A9410G A12358G T13656C A15326G T16189C C16192T T16519C
2. HQ659700(Scotland) FTDNA H100 07-DEC-2010 A93G A200G A263G 315.1C A750G T1391C A1438G A4769G A8860G A12358G G15110A A15326G T16519C

kafky
05-25-2015, 04:45 PM
Basically, I have all common mutations and none of the specific from each H100 branchs (only two no calls):

1. Kloss A263G+ 315.1C± A750G+ A1041G- A1438G+ A4769G+ A8860G+ A9410G- A12358G+ T13656C- A15326G± T16189C- C16192T- T16519C +
2. (Scotland) A93G- A200G- A263G+ 315.1C± A750G+ T1391C- A1438G+ A4769G+ A8860G+ A12358G+ G15110A- A15326G± T16519C +


There seams to be a third branch on h100. But I am not the perfect person to state this.

Kafky

kafky
01-10-2016, 07:15 PM
The underlined values do match my result.


1. Kloss ________________ A263G+ 315.1C± A750G+ A1041G- _______ A1438G+ A4769G+ A8860G+ A9410G- A12358G+ T13656C- ________A15326G± T16189C- C16192T- T16519C +
2. (Scotland) A93G- A200G- A263G+ 315.1C± A750G+ ________T1391C- A1438G+ A4769G+ A8860G+ _______A12358G+________ G15110A- A15326G±_________________T16519C +

kafky
02-27-2016, 11:42 AM
In the current Phylotree H100 is defined by A12358G which is a somewhat common mutation that has occurred independently at least twice in haplogroup H, so it is not reliable for defining a subclade of H, and the Phylotree needs to be revised for H100. If you have other extra mutations in mthap, they might help identify a new subclade of H, although you might need to test the full sequence to get a more definitive result.

Among the GenBank samples that currently define H100 there are 2 unrelated groups:

Samples from Scotland and Ireland have extra mutations at 93, 15110, 200, 1391 and 12358. I would change H100 to be defined by 93 and 15110 because there are other people who share these two mutations but who lack the mutation at 12358.

A GenBank sample from Austria has extra mutations at 1041, 9410, 12358, 13656, 16189 and 16192.

The full sequence test would determine if you are in one of these two groups or perhaps a new subclade of H.


Gait

I was looking on the 17th version if the A12358G was frequent. Only saw this mutation for H100. Have you confunded with A12356G, which is in fact quite frequent? Thanks again!

GailT
02-27-2016, 05:24 PM
Gait

I was looking on the 17th version if the A12358G was frequent. Only saw this mutation for H100. Have you confunded with A12356G, which is in fact quite frequent? Thanks again!


In my database of H* samples, which includes GenBank and the FTDNA H subclade Discovery project, A12358G occurs independently in 3 different branches of H, so H100 needs to be redefined. I have not seen any instance of A12356G in the H* samples. In some cases it is very difficult to sort the subclades of H because of parallel mutations, which is an indication that the H population expanded in size very rapidly rapidly.

Saetro
02-28-2016, 03:01 AM
The full sequence test would determine if you are in one of these two groups or perhaps a new subclade of H.

Kafky, I do not have GailT's expert knowledge of the area.
However, friends who have gone beyond their 23andMe results to do full sequence mtDNA have found very useful information.
There is only so far that limited information can take you.
You appear to have a very interesting haplotype. You can make a contribution to knowledge.
Go for it!

kafky
02-28-2016, 09:40 PM
Thanks GAit! I was checking and you're right, again. I must have mistaken the reading of the mutation number. Can you share with me which haplogroups also have the A12358G mutation? I probably could exclude all other haplogroups, since I share the mutations with other Scottish and Austrian samples, that exclusion is probable.

Saetro, yes I am thinking on a complete sequencing. 23andme has a quite good mtdna but the awful Y completes the need for more exhaustive testing. So the mtdna priority is not the most relevant, at least now. The other side, is doing the most cost effective choice, considering my very low budget available. Do you have any suggestion?

Warmest regards,

José

Saetro
02-28-2016, 11:54 PM
Saetro, yes I am thinking on a complete sequencing. ..doing the most cost effective choice, considering my very low budget available. Do you have any suggestion?


FTDNA (the only place doing full sequence mtDNA) often has discounts around US Mother's Day (8th May 2016).
Keep checking throughout April.

They often have Y-DNA discounts around US Father's Day (19 June 2016).
You can also usually find a discount on Y-DNA if you join a surname project.
Do your homework before you buy.
If you have questions about a surname or haplogroup project, ask the admins.
And see what other people have received and if it suits your needs.

If your own funds are very low and you really want this now, you might suggest friends and family contribute to this rather than a birthday gift. That works for some people.

kafky
02-29-2016, 02:08 PM
I was more thinking of full genome or exome sequencing, no pit stops...

kafky
05-08-2016, 10:07 PM
FTDNA (the only place doing full sequence mtDNA) often has discounts around US Mother's Day (8th May 2016).
Keep checking throughout April.

They often have Y-DNA discounts around US Father's Day (19 June 2016).
You can also usually find a discount on Y-DNA if you join a surname project.
Do your homework before you buy.
If you have questions about a surname or haplogroup project, ask the admins.
And see what other people have received and if it suits your needs.

If your own funds are very low and you really want this now, you might suggest friends and family contribute to this rather than a birthday gift. That works for some people.

Saetro, would you consider the WGS X2 a good option?