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  1. #1
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    Ancient I-M253 samples list

    I'm hoping that a few people here are interested in ancient I1 results. The latest Germanic aDNA paper (behind a paywall at https://www.sciencedirect.com/scienc...05440318300505) includes an elderly I1 male from Altenerding-Klettham in southern Germany. I haven't accessed the paper but assume this is the male aged 60+ from Altenerding-Klettham, buried with a spatha and other grave goods, who also features in the recent Bavaria elongated skulls paper (no Y DNA data released). We also had two I1 individuals in the recent Langobard study and I may have missed others...
    Has anyone here got an up-to-date list of ancient I1 samples, perhaps with subclades and other useful info? If someone has the data to kick things off, maybe we could post more results on this thread as they are published in future.
    Thanks
    Last edited by JonikW; 03-18-2018 at 01:23 PM.
    Living DNA's former Cautious mode:
    Wales-related ancestry: 86.8%
    Cornwall: 8%
    North England-related ancestry: 5.2%
    Y line: Peak District, England. Big Y match: Scania, Sweden; TMRCA 1,250 ybp (YFull);
    mtDNA: traces to Glamorgan, Wales
    Mother's Y: traces to Llanvair Discoed, Wales

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  3. #2
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    Thanks for sharing!

    I was unaware that the Langobard samples were I1, I assume this individual in Altenerding-Klettham was an Alemanni?

    I'm not aware of any up-to-date ancient I1 samples and their possible subclades. I hope someone has one!

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    This is what I have so far:

    StoraFörvar11 5500-5250 BC Stora Förvar cave, Stora Karlsö Island Sweden SHG pre-I1-M253 (xL121) Skoglund 2014

    BAB5 5300-4900 BC* Balatonszemes-Bagódomb Hungary LBKT_Neolithic I1-M253 Szécsényi-Nagy 2014

    RISE179 2010-1776 BC Abekĺs I Sweden Nordic_LN I1-M253 Allentoft 2015

    RISE207 1493-1302 BC Angmollan Sweden Nordic_BA I1-M253 Allentoft 2015

    RISE210 1432-1292 BC Ängamöllan Sweden Nordic_BA I1-M253? Allentoft 2015

    RISE175 1395-1132 BC Abekĺs I Sweden Nordic_BA I1-M253 Allentoft 2015

    KO_55 100-300 AD Kowalewko Poland Wielbark_Culture I1a3a1a1a-M253>DF29>Z63>BY151>S2078>S2077>Y2245>L1237 Zenczak 2017

    NO3423 550-650 AD Norton on Tees Great Britain Anglo-Saxon I1-M253 Martiniano 2016

    SZ45 600-650 AD Szolad Hungary Langobard I1a1b1-M253>DF29>Z2336>Z2337>L22 Amorim 2018

    ME_7 1000-1200 AD Markowice Poland Medieval I1a2a2a5-M253>DF29>Z58>Z59>Z2041>Z2040>Z382>Y5384 Zenczak 2017

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  7. #4
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    Quote Originally Posted by Pribislav View Post
    This is what I have so far:

    StoraFörvar11 5500-5250 BC Stora Förvar cave, Stora Karlsö Island Sweden SHG pre-I1-M253 (xL121) Skoglund 2014

    BAB5 5300-4900 BC* Balatonszemes-Bagódomb Hungary LBKT_Neolithic I1-M253 Szécsényi-Nagy 2014

    RISE179 2010-1776 BC Abekĺs I Sweden Nordic_LN I1-M253 Allentoft 2015

    RISE207 1493-1302 BC Angmollan Sweden Nordic_BA I1-M253 Allentoft 2015

    RISE210 1432-1292 BC Ängamöllan Sweden Nordic_BA I1-M253? Allentoft 2015

    RISE175 1395-1132 BC Abekĺs I Sweden Nordic_BA I1-M253 Allentoft 2015

    KO_55 100-300 AD Kowalewko Poland Wielbark_Culture I1a3a1a1a-M253>DF29>Z63>BY151>S2078>S2077>Y2245>L1237 Zenczak 2017

    NO3423 550-650 AD Norton on Tees Great Britain Anglo-Saxon I1-M253 Martiniano 2016

    SZ45 600-650 AD Szolad Hungary Langobard I1a1b1-M253>DF29>Z2336>Z2337>L22 Amorim 2018

    ME_7 1000-1200 AD Markowice Poland Medieval I1a2a2a5-M253>DF29>Z58>Z59>Z2041>Z2040>Z382>Y5384 Zenczak 2017
    The other I1 Lombard sample was from the cemetery at Collegno, northern Italy, dated 580-630CE in the paper. He was I1a3 Z79 and is labelled as sample ID number CL63.
    From the paper: "Unlike at Szólád, we find evidence of only one other individual (CL63) that is of predominant central/northern ancestry that does not belong to the major kindred unit (though our sampling of this cemetery is not as complete as Szólád)."
    Last edited by JonikW; 03-18-2018 at 03:19 PM. Reason: Bolded up haplogroup
    Living DNA's former Cautious mode:
    Wales-related ancestry: 86.8%
    Cornwall: 8%
    North England-related ancestry: 5.2%
    Y line: Peak District, England. Big Y match: Scania, Sweden; TMRCA 1,250 ybp (YFull);
    mtDNA: traces to Glamorgan, Wales
    Mother's Y: traces to Llanvair Discoed, Wales

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    Quote Originally Posted by JonikW View Post
    The other I1 Lombard sample was from the cemetery at Collegno, northern Italy, dated 580-630CE in the paper. He was I1a3 Z79 and is labelled as sample ID number CL63.
    From the paper: "Unlike at Szólád, we find evidence of only one other individual (CL63) that is of predominant central/northern ancestry that does not belong to the major kindred unit (though our sampling of this cemetery is not as complete as Szólád)."
    The problem is Z79 is downstream of I2, not I1. So something is not right with CL63, whether they've mistaken haplogroup assignment or SNP.

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  11. #6
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    Quote Originally Posted by Pribislav View Post
    The problem is Z79 is downstream of I2, not I1. So something is not right with CL63, whether they've mistaken haplogroup assignment or SNP.
    I just realised that myself... So he was either I1a3 or Z79. Has anyone checked the raw files for CL63?
    Living DNA's former Cautious mode:
    Wales-related ancestry: 86.8%
    Cornwall: 8%
    North England-related ancestry: 5.2%
    Y line: Peak District, England. Big Y match: Scania, Sweden; TMRCA 1,250 ybp (YFull);
    mtDNA: traces to Glamorgan, Wales
    Mother's Y: traces to Llanvair Discoed, Wales

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    Quote Originally Posted by JonikW View Post
    I just realised that myself... So he was either I1a3 or Z79. Has anyone checked the raw files for CL63?
    I realize that this is several months after the question was asked regarding the other I1 Lombard sample was from the cemetery at Collegno, northern Italy, dated 580-630CE in the paper, but it popped up again on the I-M253 Facebook page today.

    You're quite right - Z79 is a SNP that is part of the I2 haplogroup.

    I had a look at the supporting information for the paper at https://www.nature.com/articles/s414...-06024-4#Sec23 - if you click on Supplementary Data 3 it will bring up an excel file which has more information.

    Looking at CL63 they list him as positive for Z79. I checked the position number 17147721 and that's correct for Z79 at using the hg19 reference. However, CL63 is negative for pretty much all of the upstream I2 SNPs that I can see - negative for L460, M438, etc. Then if you go and look at the I1 SNPs, CL63 is positive for several of those, including M253 and several of those in the big block of >300 SNPs that defines I1 before branching into subclades. He's positive for DF29 and negative for L22, P109, Z63.

    My feeling is that the Z79 SNP in CL63 is a false positive read and CL63 is I1 and not I2 based on the other SNPs. But I'm not sure about their haplogroup assignments - they list P109 as I2 and also I1a3 is Z63 (which he's negative for). Perhaps they're using an earlier version of the ISOGG naming convention?

    The other I1 individual in that study SZ45 also positive for a lot of the I1 SNPs. However, if I'm reading the data correctly, they've listed said individual as derivative for both L22 and Z63 so at least one of them must be wrong. Perhaps they are making calls for SNPs based on a low number of reads.
    Last edited by deadly77; 09-13-2018 at 04:23 PM. Reason: Included reference to Lombard cemetry as it's been a few months since the original post

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    I just looked for the SZ45 information in the 2018 Amorim et al paper, but I could not find anything showing a SZ mutation path leading to L22. Maybe I missed it? Or was the information from another source?

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  17. #9
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    I believe the information is coming from raw BAM files for these aDNA individuals. At least that's what I'm gathering from reading other threads.

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  19. #10
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    Quote Originally Posted by spruithean View Post
    I believe the information is coming from raw BAM files for these aDNA individuals. At least that's what I'm gathering from reading other threads.
    That's correct, apart from STR486 mentioned at the start of this thread, which is confirmed. I read on another site on the day the paper was posted that one of the finds was an elderly I1 male. This turns out to be STR486, aged 60+ and buried with a spatha and other gear.
    Living DNA's former Cautious mode:
    Wales-related ancestry: 86.8%
    Cornwall: 8%
    North England-related ancestry: 5.2%
    Y line: Peak District, England. Big Y match: Scania, Sweden; TMRCA 1,250 ybp (YFull);
    mtDNA: traces to Glamorgan, Wales
    Mother's Y: traces to Llanvair Discoed, Wales

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