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Thread: A thread for historical and pre-historic DF19 samples ... we're up to 3, and counting

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    A thread for historical and pre-historic DF19 samples ... we're up to 3, and counting

    But two of the three just showed up this year, so maybe we're on a roll!
    Now that we have "critical mass" of a sample size larger than one, I thought I would take a tip from Bollox79's U106 observations and create a thread to keep all the finds as they (hopefully) keep coming in.

    So the first two in find order (the oldest and youngest in burial date), 6Drif23 and VK333 were found at latitudes north of Hamburg, Germany. 6Drif23 in York and VK333 on the island of Íland, Sweden. The raw data for VK333 is still embargoed. In the preprint, autosomally, he looked like a number of other Danish Viking-era samples who all had a triple-peak mix of southern, western and northern components. Culturally (burial style and carved incisors) VK333 very much looks like a Viking.

    Here is 6Drif23's autosomal G25 map:
    Quote Originally Posted by ph2ter View Post
    [FONT="][/FONT]
    Quote Originally Posted by ph2ter View Post

    [FONT="]

    [/FONT]
    ^^Culturally he and the rest of the decapitated Driffield Terrace crowd are something of a mystery. They were buried in a similar context, but it's not totally clear what was going on.

    The third sample was found buried at the Mausoleo di Augusto (Mausoleum of the Emperor Augustus and a whole host of other Roman VIPs). We can probably rule out his being a Roman VIP, and his burial date estimate overlaps the date of the Mausoleum's looting (along with the rest of Rome) by Alaric's forces in 410 AD. While his burial far more south, his autosomal map is even more north "Germanic" than 6Drif23:

    Quote Originally Posted by ph2ter View Post

    [COLOR=#FFFFFF]

    Y-SNPs: 6Drif23 is a DF88, RMPR31 is a Z302, and so far VK333 is just classified as DF19.
    DF88 and Z302 are the two main subclades of DF19 and probably date at least as far back as Beaker times, but their modern distribution doesn't look radically different. Their modern distribution looks roughly like U106, just with far fewer members.
    The two subclades may have traveled together or lived adjacently for a long time.
    Last edited by Dewsloth; 12-13-2019 at 08:13 PM.
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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    Thanks to Sheepslayer, we now know VK333's line:
    Quote Originally Posted by sheepslayer View Post
    VK333 (Oland_1028), Sweden, 885 ▒ 69 CE: R1b-DF19 > FGC11833 > S4281 > S4268 > Z17112 > FT354149 > Z17125 > Z17123 > Z29034*

    Level DF88:
    S4267 (+2), S7445 (+1), S23780 (+1)

    Level FGC11833: FGC11833 (+1)

    Level S4281: Z17109 (+2), Z17108 (+1), S4281 (+1)

    Level S4268: Z17111 (+3)

    Level Z17112:
    FGC68462 (+1)

    Level FT354149: No calls, positive downstream. Presumed positive
    Level Z27257: Z27261 (-1), Z27260 (-1)
    Level S17075: S17075 (-1)
    Level PH3649: S4273 (-1), S4276 (-1), S4288 (-1), Z39822 (-1)

    Level Z21380: No calls, negative downstream

    Level Z17125:
    FT37310 (+1)

    Level Z17123: No calls, positive downstream. Presumed positive

    Level Z29034: Z29036 (+2), Z29040 (+2), Z29037 (+2), Z29039 (+1)

    Negative for FT116675 (1 read) and all downstream. Very negative for BY56504
    You can now see VK333 and 6DRIF23 on the Big Tree:
    https://www.ytree.net/DisplayTree.php?blockID=183
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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    And finally #4 appears, and he was worth the wait. Alex Williamson identified one of the Dutch Bell Beaker samples as a DF19!

    Alex just identified I5748 as a DF19>Z302
    Noord-Holland
    aDNA: I5748
    MDKA Birth: Netherlands
    Bell Beaker, PRJEB23635. 2579-2211 calBCE (3945▒55 BP, GrN-6650C). Noord-Holland, Oostwoud, De Tuithoorn Netherlands.
    Tree Position
    R-P312/S116 > DF19/S232 > Z302/S233 > Exact position not yet finalized.
    https://www.ytree.net/SNPinfoForPers...personID=12062

    First DF19 to be found dated to earlier than 200AD
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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    I looked at the BAM, I'm afraid I5748 is either Z302* or some undiscovered block split because he has one negative read each for two variants denoting both branches directly downstream of Z302.

    My notes:
    I5748.png

    Couldn't find any reads on level Z302
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    Quote Originally Posted by sheepslayer View Post
    I looked at the BAM, I'm afraid I5748 is either Z302* or some undiscovered block split because he has one negative read each for two variants denoting both branches directly downstream of Z302.

    My notes:
    I5748.png

    Couldn't find any reads on level Z302
    Thanks! It's kinda crazy, we just jumped back from 200 AD to almost butting up against the P312 event horizon: P312's TMRCA at yfull is 4500ybp, DF19 (and both main subclades, DF88 and Z302) formed around 4500 ybp along with P312's other "sons"
    At ~3945ybp, this burial is only about 600 years after Z302* was formed.
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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    Quote Originally Posted by Dewsloth View Post
    And finally #4 appears, and he was worth the wait.
    Indeed he was worth it. Olalde et. al., 2018 Supplementary Information (at https://www.nature.com/articles/nature25738#Sec21) refer to the original excavation of I5748 where he was called Skeleton 575, and that work is summarised in a very comprehensive paper by Fokkens et. al. 2017, available for download at https://scholarlypublications.univer...dle/1887/57381. My quiet weekend just became a little busier.

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    df19map.PNG

    ^^ York, England; Vickleby, Sweden; and Oostwoud, Netherlands.
    So three of the four ancient DF19 finds are from latitudes even with or north of Berlin, and the fourth (RMPR31 buried down in Rome ~400AD) looks more (modern) Norwegian than the rest...

    Distance to: ITA_Rome_Late_Antiquity:RMPR31
    0.02240159 Norwegian
    0.02290643 Dutch
    0.02295681 Danish
    0.02304767 French_Pas-de-Calais
    0.02341117 Afrikaner
    0.02345321 Swedish
    0.02382061 Welsh
    0.02412413 German
    0.02468599 English
    0.02499629 Icelandic


    Distance to: Bell_Beaker_NLD:I5748
    0.02355857 Norwegian
    0.02409423 Icelandic
    0.02440895 Irish
    0.02451191 Danish
    0.02470650 Shetlandic
    0.02544114 Scottish
    0.02549502 Swedish
    0.02567693 Dutch
    0.02569311 Orcadian
    0.02630714 Welsh


    Mind you I'm not saying R31 is Norwegian, I'm just saying his burial location can't be a good indication of his ethnic or geographic origin.

    Also I5748 might be the first DF19 sample to be buried near where he grew up.
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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    Just for reference. These are in chronological order, but it's also order of Barcin percentage (scaled)
    Code:
    Target---              Barcin_N--WHG--Yamnaya_Samara
    Bell_Beaker_NLD:I5748	       23.4	12.8	63.8
    England_Roman:6DT23	       37.2	14.6	48.2
    ITA_Rome_Late_Antiquity:RMPR31	  38.8	17.6	43.6
    VK2020_SWE_Oland_VA:VK333	  44.8	11.0	44.2
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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    There will be some updates from the upcoming paper on Bronze Age Britain: "Large-Scale Migration into Southern Britain During the Middle to Late Bronze Age"
    https://www.ebi.ac.uk/ena/browser/vi...891?show=reads

    In the 800+ BA and IA samples from the study, there are three that look interesting but may not be confirmed until they can really be analyzed:

    Yleaf results:
    I26830 R1b1a1b1a1a2e R-DF19*(xL644,L1199,S233)
    I16182 R1b1a1b1a1a2e1b R-L1199

    SNIPSA results:
    I17607:Result (81.8% 39 -1 +5): R-Y6234
    I17607:R1b -> R-L754 -> R-L389 -> R-P297 -> R-M269 -> R-L23 -> R-L51 -> R-L52 -> R-L151 -> R-P312 -> R-DF19 -> R-DF88 -> R-Y3096 -> R-Y6234 (ISOGG: )
    I17607:Extra: Y19636:T FGC42546:T MF87426:A V6772/BY115803:T Y8743/FGC22598:T

    [snipsa thinks I16182 is actually not DF19]
    I16182:Result (81.3% 444 -2 +95): R-L2
    I16182:R1b -> R-L754 -> R-L389 -> R-P297 -> R-M269 -> R-L23 -> R-L51 -> R-L52 -> R-L151 -> R-P312 -> R-U152 -> R-L2 (ISOGG: R1b1a1b1a1a2b1)

    no snipsa data yet for I26830 due to download issues. We won't know more about the samples until the paper is formally published, apparently.

    Stay tuned!
    Last edited by Dewsloth; 11-02-2021 at 04:54 PM.
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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  19. #10
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    We still don't know much about them, but here are our two new apparent DF19s from the upcoming Celtic paper:

    Quote Originally Posted by ph2ter View Post
    Thanks!

    [FONT="]
    [/FONT]
    R1b>M269>L23>L51>L11>P312>DF19>DF88>FGC11833 >S4281>S4268>Z17112>FT354149

    Ancestors: Francis Cooke (M223/I2a2a) b1583; Hester Mahieu (Cooke) (J1c2 mtDNA) b.1584; Richard Warren (E-M35) b1578; Elizabeth Walker (Warren) (H1j mtDNA) b1583;
    John Mead (I2a1/P37.2) b1634; Rev. Joseph Hull (I1, L1301+ L1302-) b1595; Benjamin Harrington (M223/I2a2a-Y5729) b1618; Joshua Griffith (L21>DF13) b1593;
    John Wing (U106) b1584; Thomas Gunn (DF19) b1605; Hermann Wilhelm (DF19) b1635

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