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Thread: Can Yfull use my low yield Nebula BAM file?

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  1. #1
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    Can Yfull use my low yield Nebula BAM file?

    Thanks.
    Eurogenes K-13
    1 Belorussian + Italian_Jewish + North_German + Southeast_English @ 1.607897
    2 Estonian_Polish + Italian_Jewish + North_German + Southeast_English @ 1.623309
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    5 Ashkenazi + Belorussian + Southeast_English + West_German @ 1.696746
    6 Belorussian + Danish + Italian_Jewish + West_German @ 1.701075

  2. #2
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    If you mean the 0.4x one then the answer is no.

  3. The Following 4 Users Say Thank You to teepean47 For This Useful Post:

     deadly77 (11-28-2020),  JMcB (11-28-2020),  Mixed (11-28-2020),  Oleg (Rus) (11-29-2020)

  4. #3
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    Agree with teepean47 - the 0.4x one will be so full of no calls and false positives it won't be worth it.

    If not the 0.4x, YFull's order page says "Technical requirements: .BAM file; depth of coverage min 15X; read length min 100 bp" so use that as your guide.
    Haplogroup I1 Ancient DNA Samples Map: Hidden Content

  5. The Following 3 Users Say Thank You to deadly77 For This Useful Post:

     Jatt1 (12-03-2020),  JMcB (11-28-2020),  Mixed (11-28-2020)

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    Quote Originally Posted by Mixed View Post
    Thanks.
    When you say low yield, do you mean your data has a low % of reads mappable to the human reference (due to contamination)?
    YFull: YF72440 (FTDNA - IN41220)

    Ancestral Haplos (Punjabi Jatt):
    * Father: R2-M479 > M124 > V1180 > SK2142 > Y1379 > Y1383 > Y154920* (xZ6135) - M5a1a-G9064A (185G)
    * Maternal Uncle: R1b-M343 > M269 > Z2103 > Z2109 > Y14416 > Y35099 > Y84821 - U7a3a-A9852G > G6150A > C15433T
    * MGMs MGF: R1a-M420 > M198 > Z93 > L657 > Y7 - ?

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     Jatt1 (11-29-2020)

  8. #5
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    Quote Originally Posted by aaronbee2010 View Post
    When you say low yield, do you mean your data has a low % of reads mappable to the human reference (due to contamination)?
    No. It was the cheaper test.
    Eurogenes K-13
    1 Belorussian + Italian_Jewish + North_German + Southeast_English @ 1.607897
    2 Estonian_Polish + Italian_Jewish + North_German + Southeast_English @ 1.623309
    3 Belorussian + Italian_Jewish + Norwegian + West_German @ 1.652029
    4 Danish + Italian_Jewish + Lithuanian + West_German @ 1.692531
    5 Ashkenazi + Belorussian + Southeast_English + West_German @ 1.696746
    6 Belorussian + Danish + Italian_Jewish + West_German @ 1.701075

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