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Thread: Genetic Genealogy & Ancient DNA in the News (DISCUSSION ONLY)

  1. #3201
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    Quote Originally Posted by rozenfeld View Post
    Weird paper, they didn't even get mtDNA:

    https://onlinelibrary.wiley.com/doi/...002/ajpa.23996

    Genome‐wide SNP typing of ancient DNA: Determination of hair and eye color of Bronze Age humans from their skeletal remains

    Nicole Schmidt, Katharina Schücker, Ina Krause, Thilo Dörk, Michael Klintschar, Susanne Hummel

    First published: 20 January 2020
    https://doi.org/10.1002/ajpa.23996

    Abstract

    Objective

    A genome‐wide high‐throughput single nucleotide polymorphism (SNP) typing method was tested with respect of the applicability to ancient and degraded DNA. The results were compared to mini‐sequencing data achieved through single base extension (SBE) typing. The SNPs chosen for the study allow to determine the hair colors and eye colors of humans.

    Material and methods

    The DNA samples were extracted from the skeletal remains of 59 human individuals dating back to the Late Bronze Age. The 3,000 years old bones had been discovered in the Lichtenstein Cave in Lower Saxony, Germany. The simultaneous typing of 24 SNPs for each of the ancient DNA samples was carried out using the 192.24 Dynamic Array™ by Fluidigm®.

    Results

    Thirty‐eight of the ancient samples (=64%) revealed full and reproducible SNP genotypes allowing hair and eye color phenotyping. In 10 samples (=17%) at least half of the SNPs were unambiguously determined, in 11 samples (=19%) the SNP typing failed. For 23 of the 59 individuals, a comparison of the SNP typing results with genotypes from an earlier performed SBE typing approach was possible. The comparison confirmed the full concordance of the results for 90% of the SNP typings. In the remaining 10% allelic dropouts were identified.

    Discussion

    The high genotyping success rate could be achieved by introducing modifications to the preamplification protocol mainly by increasing the DNA input and the amplification cycle number. The occurrence of allelic dropouts indicates that a further increase of DNA input to the preamplification step is desirable.
    Very strange publication indeed.
    No autosomal comparison to other known cultutes, no happlogroups, no supplementary info about archeology.

    Quote Originally Posted by etrusco View Post
    They were 15 persons.
    12 were I2b
    1 R1b
    1 R1a
    Would be nice to see more details. it is always interesting to see, where the surviving I2 branches are going to show up. I2b in 2006 - was that P37 or M436?

    DATA AVAILABILITY STATEMENT
    The data that support the findings of this study are available from the corresponding author upon reasonable request
    What is that? Why didn't they just upload data to European Nucleotide Archive? And why do they write about "reasonable" request, they aren't going to share the results just with everyone?

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    Quote Originally Posted by etrusco View Post
    They were 15 persons.
    12 were I2b
    1 R1b
    1 R1a
    So, I guess there will be paper with more thorough analysis of these remains? if so, when can we expect it?

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    If you look at the other papers published by the senior author you will see they haven’t specialized in ancient DNA studies. So probably don’t know the culture. Somebody probably came to them with specimens asking for technical help. “What would be a good first paper?” Been in those situations.

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    Quote Originally Posted by rozenfeld View Post
    So, I guess there will be paper with more thorough analysis of these remains? if so, when can we expect it?
    These are old results from 2006.
    https://en.wikipedia.org/wiki/Lichtenstein_Cave
    And on wikipedia you can find a link to original document, 2006 (in German):
    http://webdoc.sub.gwdg.de/diss/2006/schilz/schilz.pdf

    I'm interested in getting the bam files and checking for the subclade.

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  9. #3205
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    Quote Originally Posted by rozenfeld View Post
    I am not sure whether it was posted before:

    https://scienceinpoland.pap.pl/en/ne...say-scientists

    Four Warriors Buried in 11th Century Tombs in Pomerania Came From Scandinavia, say Scientists

    Samples of some of the remains were taken and sent for strontium isotope studies and genetic analyses which enabled the scientists to determine the origin, migration directions, kinship and appearance of the deceased.

    Some more details may be found here https://www.academia.edu/41674465/ at pages 572 and 574.

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  11. #3206
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    ^^^
    That book also says that 58A was most likely the mother of 58B, but 58A has local strontium isotopes and 58B non-local. They have same mtDNA. 58B was buried with his mom but he died years later (the coffin was opened, and he was added to the grave).

    Looks like 58B emigrated from Poland as a child (maybe with his father or with both parents) and came back as an adult, hence non-local isotopes.

    Warriors 58B and 47 have non-local strontium isotopes (they most probably spent their childhood in Denmark, or elsewhere) but local very typically West Slavic Y-DNA haplogroups (and probably also autosomally they will turn out Polish-like).

    The mother (58A) has local North Polish isotopes.

    =====

    Scan from Waldemar:



    Waldemar has the same Y-HG (R1a-L1029) as these two warriors, and this is also the Y-HG of my maternal grandpa as it turns out (based on Y111):

    Last edited by Tomenable; 01-27-2020 at 11:24 PM.

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    Quote Originally Posted by Tomenable View Post
    ^^^
    That book also says that 58A was most likely the mother of 58B, but 58A has local strontium isotopes and 58B non-local. They have same mtDNA. 58B was buried with his mom but he died years later (the coffin was opened, and he was added to the grave).

    Looks like 58B emigrated from Poland as a child (maybe with his father or with both parents) and came back as an adult, hence non-local isotopes.

    Warriors 58B and 47 have non-local strontium isotopes (they most probably spent their childhood in Denmark, or elsewhere) but local very typically West Slavic Y-DNA haplogroups (and probably also autosomally they will turn out Polish-like).

    The mother (58A) has local North Polish isotopes.

    =====

    Scan from Waldemar:



    Waldemar has the same Y-HG (R1a-L1029) as these two warriors, and this is also the Y-HG of my maternal grandpa as it turns out (based on Y111):

    Hmm T2b23? I am T2b2b... one of these guys is also U106+...?

    Edit: grave 35 is U106+ - are any Bam files available for these guys?
    Last edited by Bollox79; 01-28-2020 at 02:17 AM.
    Y-DNA: 5th GGF Capt. Johann Martin Weber, 1st PA Rifles, Rev. War, b. 1739 in Germany, d. 1804 Paxton, PA. : R1b-U106-DF98 - S4004 - FGC14817(5 SNPs) with 6drif-3 soldier of York! Pennsylvania Scots Irish/German frontiersmen/colonial vets.

    mtDNA: 3rd GGM Bridget O'Danagher/O'Kennedy/MacEgan, Lorrha/Dorrha, Tipp - T2b2b. Mother's dad The O'Dwyer - O'Dwyers of Kilnamanagh/Ballagh Maguires/Fermanagh matching descendants of Irish exiles in Europe.

    Hebridean/Highland Scots both sides ;-).

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  15. #3208
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    delete
    Y-DNA: 5th GGF Capt. Johann Martin Weber, 1st PA Rifles, Rev. War, b. 1739 in Germany, d. 1804 Paxton, PA. : R1b-U106-DF98 - S4004 - FGC14817(5 SNPs) with 6drif-3 soldier of York! Pennsylvania Scots Irish/German frontiersmen/colonial vets.

    mtDNA: 3rd GGM Bridget O'Danagher/O'Kennedy/MacEgan, Lorrha/Dorrha, Tipp - T2b2b. Mother's dad The O'Dwyer - O'Dwyers of Kilnamanagh/Ballagh Maguires/Fermanagh matching descendants of Irish exiles in Europe.

    Hebridean/Highland Scots both sides ;-).

  16. #3209
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    Quote Originally Posted by Waldemar View Post
    2x R1a-L1029 (grave 58B - 1140±30 BP, grave 47 - 1090±30 BP), strontium isotope ratios - both non-local, possibly from Scandinavia (but from different regions), grave 58A - mother of 58B with local North Polish strontium isotope ratios, 47 could be a son of 58B

    1x R1b-FGC11293 (grave 43 - 1100±30 BP) - non-local

    1x R1b-U106 (grave 35 - 1065±30 BP) - non-local

    Age distribution of buried:
    58B - maturus/senilis
    47 - maturus
    43 - maturus
    35 - maturus/senilis



    Grave 58


    Other R1a-L1029 samples from "Population genomics of the Viking world", e.g. from Funen, Denmark - https://anthrogenica.com/showthread....l=1#post583229
    Last edited by Waldemar; 01-29-2020 at 10:17 AM.

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